pronex-size selective purification system ng2001 (Promega)
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pronex-size selective purification system ng2001
Pronex Size Selective Purification System Ng2001, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pronex+size-selective+purification+system+ng2001/pronex+size+selective+purification+system/pmc12204849-202-18-18
Average 90 stars, based on 1 article reviews
Pronex Size Selective Purification System Ng2001, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pronex+size-selective+purification+system+ng2001/pronex+size+selective+purification+system/pmc12204849-202-18-18
Average 90 stars, based on 1 article reviews
pronex-size selective purification system ng2001 - by Bioz Stars,
2026-09
90/100 stars
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Polymerase Chain Reaction:Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: The size-selected library was amplified for six cycles using Article Title: hGRAD: A versatile “one-fits-all” system to acutely deplete RNA binding proteins from condensates Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode ( ). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode (Buchbender et al., 2020). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: Single-nucleotide-resolution genomic maps of O6-methylguanine from the glioblastoma drug temozolomide. Article Snippet: ProNex Size-Selective Purification System (NG2001), ECL Western Blotting Substrate (W1001), Article Title: An integrated pipeline for mammalian genetic screening Article Snippet: Article Title: Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering. Article Snippet: PCR products were purified ( Article Title: Arid5a uses disordered extensions of its core ARID domain for distinct DNA- and RNA-recognition and gene regulation. Article Snippet: 9) and then size-selected using the Purification:Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: The size-selected library was amplified for six cycles using Article Title: hGRAD: A versatile “one-fits-all” system to acutely deplete RNA binding proteins from condensates Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode ( ). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode (Buchbender et al., 2020). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: Single-nucleotide-resolution genomic maps of O6-methylguanine from the glioblastoma drug temozolomide. Article Snippet: ProNex Size-Selective Purification System (NG2001), ECL Western Blotting Substrate (W1001), Article Title: An integrated pipeline for mammalian genetic screening Article Snippet: Article Title: Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering. Article Snippet: PCR products were purified ( Article Title: Arid5a uses disordered extensions of its core ARID domain for distinct DNA- and RNA-recognition and gene regulation. Article Snippet: 9) and then size-selected using the Western Blot:Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: The size-selected library was amplified for six cycles using Article Title: hGRAD: A versatile “one-fits-all” system to acutely deplete RNA binding proteins from condensates Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode ( ). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode (Buchbender et al., 2020). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: Single-nucleotide-resolution genomic maps of O6-methylguanine from the glioblastoma drug temozolomide. Article Snippet: ProNex Size-Selective Purification System (NG2001), ECL Western Blotting Substrate (W1001), Article Title: An integrated pipeline for mammalian genetic screening Article Snippet: Article Title: Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering. Article Snippet: PCR products were purified ( Article Title: Arid5a uses disordered extensions of its core ARID domain for distinct DNA- and RNA-recognition and gene regulation. Article Snippet: 9) and then size-selected using the Extraction:Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: The size-selected library was amplified for six cycles using Article Title: hGRAD: A versatile “one-fits-all” system to acutely deplete RNA binding proteins from condensates Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode ( ). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode (Buchbender et al., 2020). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: Single-nucleotide-resolution genomic maps of O6-methylguanine from the glioblastoma drug temozolomide. Article Snippet: ProNex Size-Selective Purification System (NG2001), ECL Western Blotting Substrate (W1001), Article Title: An integrated pipeline for mammalian genetic screening Article Snippet: Article Title: Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering. Article Snippet: PCR products were purified ( Article Title: Arid5a uses disordered extensions of its core ARID domain for distinct DNA- and RNA-recognition and gene regulation. Article Snippet: 9) and then size-selected using the Reverse Transcription:Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: The size-selected library was amplified for six cycles using Article Title: hGRAD: A versatile “one-fits-all” system to acutely deplete RNA binding proteins from condensates Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode ( ). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode (Buchbender et al., 2020). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: Single-nucleotide-resolution genomic maps of O6-methylguanine from the glioblastoma drug temozolomide. Article Snippet: ProNex Size-Selective Purification System (NG2001), ECL Western Blotting Substrate (W1001), Article Title: An integrated pipeline for mammalian genetic screening Article Snippet: Article Title: Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering. Article Snippet: PCR products were purified ( Article Title: Arid5a uses disordered extensions of its core ARID domain for distinct DNA- and RNA-recognition and gene regulation. Article Snippet: 9) and then size-selected using the Ligation:Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: The size-selected library was amplified for six cycles using Article Title: hGRAD: A versatile “one-fits-all” system to acutely deplete RNA binding proteins from condensates Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode ( ). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode (Buchbender et al., 2020). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: Single-nucleotide-resolution genomic maps of O6-methylguanine from the glioblastoma drug temozolomide. Article Snippet: ProNex Size-Selective Purification System (NG2001), ECL Western Blotting Substrate (W1001), Article Title: An integrated pipeline for mammalian genetic screening Article Snippet: Article Title: Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering. Article Snippet: PCR products were purified ( Article Title: Arid5a uses disordered extensions of its core ARID domain for distinct DNA- and RNA-recognition and gene regulation. Article Snippet: 9) and then size-selected using the Amplification:Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: The size-selected library was amplified for six cycles using Article Title: hGRAD: A versatile “one-fits-all” system to acutely deplete RNA binding proteins from condensates Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode ( ). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode (Buchbender et al., 2020). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: Single-nucleotide-resolution genomic maps of O6-methylguanine from the glioblastoma drug temozolomide. Article Snippet: ProNex Size-Selective Purification System (NG2001), ECL Western Blotting Substrate (W1001), Article Title: An integrated pipeline for mammalian genetic screening Article Snippet: Article Title: Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering. Article Snippet: PCR products were purified ( Article Title: Arid5a uses disordered extensions of its core ARID domain for distinct DNA- and RNA-recognition and gene regulation. Article Snippet: 9) and then size-selected using the Virus:Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: The size-selected library was amplified for six cycles using Article Title: hGRAD: A versatile “one-fits-all” system to acutely deplete RNA binding proteins from condensates Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode ( ). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode (Buchbender et al., 2020). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: Single-nucleotide-resolution genomic maps of O6-methylguanine from the glioblastoma drug temozolomide. Article Snippet: ProNex Size-Selective Purification System (NG2001), ECL Western Blotting Substrate (W1001), Article Title: An integrated pipeline for mammalian genetic screening Article Snippet: Article Title: Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering. Article Snippet: PCR products were purified ( Article Title: Arid5a uses disordered extensions of its core ARID domain for distinct DNA- and RNA-recognition and gene regulation. Article Snippet: 9) and then size-selected using the Recombinant:Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: The size-selected library was amplified for six cycles using Article Title: hGRAD: A versatile “one-fits-all” system to acutely deplete RNA binding proteins from condensates Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode ( ). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode (Buchbender et al., 2020). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: Single-nucleotide-resolution genomic maps of O6-methylguanine from the glioblastoma drug temozolomide. Article Snippet: ProNex Size-Selective Purification System (NG2001), ECL Western Blotting Substrate (W1001), Article Title: An integrated pipeline for mammalian genetic screening Article Snippet: Article Title: Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering. Article Snippet: PCR products were purified ( Article Title: Arid5a uses disordered extensions of its core ARID domain for distinct DNA- and RNA-recognition and gene regulation. Article Snippet: 9) and then size-selected using the SYBR Green Assay:Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: The size-selected library was amplified for six cycles using Article Title: hGRAD: A versatile “one-fits-all” system to acutely deplete RNA binding proteins from condensates Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode ( ). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode (Buchbender et al., 2020). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: Single-nucleotide-resolution genomic maps of O6-methylguanine from the glioblastoma drug temozolomide. Article Snippet: ProNex Size-Selective Purification System (NG2001), ECL Western Blotting Substrate (W1001), Article Title: An integrated pipeline for mammalian genetic screening Article Snippet: Article Title: Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering. Article Snippet: PCR products were purified ( Article Title: Arid5a uses disordered extensions of its core ARID domain for distinct DNA- and RNA-recognition and gene regulation. Article Snippet: 9) and then size-selected using the Lysis:Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: The size-selected library was amplified for six cycles using Article Title: hGRAD: A versatile “one-fits-all” system to acutely deplete RNA binding proteins from condensates Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode ( ). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode (Buchbender et al., 2020). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: Single-nucleotide-resolution genomic maps of O6-methylguanine from the glioblastoma drug temozolomide. Article Snippet: ProNex Size-Selective Purification System (NG2001), ECL Western Blotting Substrate (W1001), Article Title: An integrated pipeline for mammalian genetic screening Article Snippet: Article Title: Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering. Article Snippet: PCR products were purified ( Article Title: Arid5a uses disordered extensions of its core ARID domain for distinct DNA- and RNA-recognition and gene regulation. Article Snippet: 9) and then size-selected using the Plasmid Preparation:Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: The size-selected library was amplified for six cycles using Article Title: hGRAD: A versatile “one-fits-all” system to acutely deplete RNA binding proteins from condensates Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode ( ). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode (Buchbender et al., 2020). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: Single-nucleotide-resolution genomic maps of O6-methylguanine from the glioblastoma drug temozolomide. Article Snippet: ProNex Size-Selective Purification System (NG2001), ECL Western Blotting Substrate (W1001), Article Title: An integrated pipeline for mammalian genetic screening Article Snippet: Article Title: Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering. Article Snippet: PCR products were purified ( Article Title: Arid5a uses disordered extensions of its core ARID domain for distinct DNA- and RNA-recognition and gene regulation. Article Snippet: 9) and then size-selected using the Gel Extraction:Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: The size-selected library was amplified for six cycles using Article Title: hGRAD: A versatile “one-fits-all” system to acutely deplete RNA binding proteins from condensates Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode ( ). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode (Buchbender et al., 2020). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: Single-nucleotide-resolution genomic maps of O6-methylguanine from the glioblastoma drug temozolomide. Article Snippet: ProNex Size-Selective Purification System (NG2001), ECL Western Blotting Substrate (W1001), Article Title: An integrated pipeline for mammalian genetic screening Article Snippet: Article Title: Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering. Article Snippet: PCR products were purified ( Article Title: Arid5a uses disordered extensions of its core ARID domain for distinct DNA- and RNA-recognition and gene regulation. Article Snippet: 9) and then size-selected using the Cloning:Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: The size-selected library was amplified for six cycles using Article Title: hGRAD: A versatile “one-fits-all” system to acutely deplete RNA binding proteins from condensates Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode ( ). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode (Buchbender et al., 2020). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: Single-nucleotide-resolution genomic maps of O6-methylguanine from the glioblastoma drug temozolomide. Article Snippet: ProNex Size-Selective Purification System (NG2001), ECL Western Blotting Substrate (W1001), Article Title: An integrated pipeline for mammalian genetic screening Article Snippet: Article Title: Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering. Article Snippet: PCR products were purified ( Article Title: Arid5a uses disordered extensions of its core ARID domain for distinct DNA- and RNA-recognition and gene regulation. Article Snippet: 9) and then size-selected using the Sequencing:Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: The size-selected library was amplified for six cycles using Article Title: hGRAD: A versatile “one-fits-all” system to acutely deplete RNA binding proteins from condensates Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode ( ). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode (Buchbender et al., 2020). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: Single-nucleotide-resolution genomic maps of O6-methylguanine from the glioblastoma drug temozolomide. Article Snippet: ProNex Size-Selective Purification System (NG2001), ECL Western Blotting Substrate (W1001), Article Title: An integrated pipeline for mammalian genetic screening Article Snippet: Article Title: Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering. Article Snippet: PCR products were purified ( Article Title: Arid5a uses disordered extensions of its core ARID domain for distinct DNA- and RNA-recognition and gene regulation. Article Snippet: 9) and then size-selected using the Software:Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: The size-selected library was amplified for six cycles using Article Title: hGRAD: A versatile “one-fits-all” system to acutely deplete RNA binding proteins from condensates Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode ( ). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: hGRAD: A versatile "one-fits-all" system to acutely deplete RNA binding proteins from condensates. Article Snippet: RNA was purified using neutral phenol/chloroform/isoamylalcohol (AM9722; Ambion) followed by chloroform (39554.02; Serva) extraction and reverse transcribed using SuperScript III (18080-044; Life Technologies). cDNA was cleaned up using MyONE Silane beads (37002D; Life Technologies) followed by ligation of a second adapter containing a bipartite (5 + 4-nt) unique molecular identifier (UMI) as well as a 6-nt experimental barcode (Buchbender et al., 2020). iCLIP2 libraries were preamplified with six PCR cycles using short primers (P5Solexa_short and P3Solexa_short) and then size-selected using the Article Title: Single-nucleotide-resolution genomic maps of O6-methylguanine from the glioblastoma drug temozolomide. Article Snippet: ProNex Size-Selective Purification System (NG2001), ECL Western Blotting Substrate (W1001), Article Title: An integrated pipeline for mammalian genetic screening Article Snippet: Article Title: Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering. Article Snippet: PCR products were purified ( Article Title: Arid5a uses disordered extensions of its core ARID domain for distinct DNA- and RNA-recognition and gene regulation. Article Snippet: 9) and then size-selected using the |